University of Oslo

Research Group: Tuula Nyman/ Proteomics

Field: Innate immunity, proteomics, biological mass spectrometry

Expertise/Competence: The group has long-standing expertise in using advanced proteomics tools to characterize innate immune cell activation with different stimuli including extensive studies on virus-infected human primary macrophages. The groups research focus is on elucidating molecular mechanisms on how viral infection activates host cells, including extensive phosphoproteomics studies on host response. They further characterize immune system activation with focus on global protein secretion and extracellular vesicle secretion and proteomics applied to clinical research.

Infrastructure: The groups proteomics laboratory is well equipped for modern, advanced proteomics studies including three high resolution LC-MS/MS instruments and all software needed for data analysis. Nyman is Project manager for National network of Advanced Proteomics Infrastructure in Norway (https://www.napi.uio.no/) and the COMBINE consortium also has direct access to other, complementary MS instrumentation and added expertise in proteomics data analysis through NAPI.

Role within COMBINE

The group will perform advanced proteomic analysis of MARV-infected host cell samples to identify signaling cascades activated by MARV-cell binding. The quantitative study includes global characterization of post-translational modifications (phosphorylation, ubiquitination) of host cells upon activation of functional receptors. Data will be integrated with interactomics and PTM signatures.